TRAINING

Seminar 1: Imaging and Microscopy

Content:

This course gives an overview on techniques of transmission, scattering and fluorescence microscopy including some biomedical applications, e.g. detection of cancer and other diseases. Methods of 3D microscopy, in particular Confocal Laser Scanning Microscopy (CLSM), Structured Illumination Microscopy (SIM) and Light Sheet based Fluorescence Microscopy (LSFM) are in the focus of this presentation, but also methods with high spectral and temporal resolution (Spectral Imaging, Fluorescence Lifetime) are emphasized. Some methods are combined with further techniques, e.g. axial tomography or laser micromanipulation.

Learning objectives include

• techniques of transmission, scattering and fluorescence microscopy;
• techniques of spectral imaging and fluorescence lifetime imaging (FLIM);
• axial tomography;
• laser micromanipulation
• biomedical applications: fluorescence diagnosis, drug response

Prof. Dr. Herbert Schneckenburger

Institute of Applied Research
Aalen University, Germany

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Herbert Schneckenburger is a professor of Physics, Optics and Biophotonics at Aalen University and a private lecturer of the Medical Faculty of the University of Ulm. He received his PhD in Physics from the University of Stuttgart in 1979 and his habilitation in Biomedical Technology from the University of Ulm in 1992. His research is focused on various fields of Biomedical Optics including optical microscopy and time-resolved laser spectroscopy. In the period of 1979-2017 Prof. Schneckenburger published more than 260 scientific articles, received 6 patents and managed and performed 34 projects with third-party funding. He is continuously active as an international expert in his field and co-organized in 2014 the International Conference on Laser Applications in Life Sciences with more than 300 participants.

Seminar 2: Hyperspectral Imaging in Industry

Content:

• Instrumentation
• Hyperspectral data and preprocessing
• Feature based processing
• Chemical Color Imaging
• Industrial application
• Modular hyperspectral processing

DI (FH) Markus Burgstaller

CEO, Head of R&D at
Perception Park GmbH

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Markus Burgstaller received his M.S. degree in electronics from the Carinthia University of Applied Science in 2002. After 2 years in the field of robot vision at Joanneum Research GmbH as well as freelancer, he started with Hyperspectral Imaging at EVK DI Kerschhaggl GmbH in 2006. After development of the first smart hyperspectral camera system he led the hyperspectral product development and later on the R&D team at EVK.

In 2012 Markus founded Perception Park with the aim to introduce the high potential hyperspectral technology to the broad industrial market. Perception Park is focused solely on data processing solutions based on the first generic, intuitive understandable and free configurable data processing platform for the industrial application of hyperspectral imaging.

Seminar 3: Hyperspectral Imaging - Problems and Solutions from a Statistical Perspective

Content:
This talk will provide an overview on a few selected statistical problems and their possible solutions in the context of hyperspectral imaging. Among the discussed topics are:

• Imaging and the curse of dimensionality
• Spectral descriptors and chemical knowledge
• How to deal with noise, spikes and baseline drift
• Classification and segmentation: improving the performance of classifiers
• Detecting particles and automatically determining their parameters

Prof. Dr. Hans Lohninger

Institute of Chemical Technologies and Analytics
Vienna University of Technology

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Trainer profile:

Hans Lohninger is professor of chemometrics at the Vienna University of Technology teaching chemometrics and statistics. His main interests in the field of hyperspectral imaging are statistical questions concerning the processing and interpretation of hyperspectral data. Apart from theoretical problems regarding the extraction of information he is also working on algorithms for combining and analyzing hyperspectral images obtained from different spectroscopic sources ("multisensor imaging").  Hans Lohninger is founder, owner and manager of Epina GmbH, a software development company which puts its focus on the development of scientific and engineering software. One of the packages developed by Epina is ImageLab, a hyperspectral imaging tool supporting multisensor imaging.

Seminar 4: Hyperspectral imaging applied in the food industry: Instrumentation, applications, and data analysis

Content:

In this lecture, researchers from Nofima will share insights from several years of hyperspectral imaging and spectroscopy research related to food. The lecture will cover multiple types of illumination: diffuse reflectance, interactance and UV light for fluorescence. Applications to different food types (e.g. fish, meat, dairy, vegetables) and for different characterization purposes (e.g., estimating food constituents or quality attributes, detecting defects, etc.) will be discussed. Relevant data analysis techniques, (e.g. PCA, PLS, classification, spatial filtering) will also be presented. The lecture will demonstrate the potential for industrial applications through a wide set of relevant examples.

Dr. Karsten Heia; Dr. Kate Washburn; Dr. Martin H. Skjelvareid

• Dr. Karsten Heia, senior researcher, Nofima
• Dr. Kate Washburn, researcher, Nofima
• Dr. Martin H. Skjelvareid, researcher, Nofima

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Trainer Profiles:

Dr. Karsten Heia obtained his PhD in physics from University of Tromsø, Norway, in 1996. Since 1996 he has been working with rapid instrumental non-destructive techniques for seafood quality at Nofima, Norway. His research interests include software development for hyperspectral image analysis, detection of food defects, and estimation of food quality attributes.

Dr. Kate Washburn obtained her B.S. in Physics from MIT and a PhD in Physics from the University of Wellington. She is an applied physicist with experience in many types of spectroscopy and data analysis techniques. Kate worked for several years as an industrial researcher in the petroleum industry before switching to food technology.

Dr. Martin H. Skjelvareid obtained his PhD in physics at the University of Tromsø, Norway, in 2012. Since then he has been working with hyspespectral imaging, image analysis, and multivariate statistics at Nofima, Norway. His current research interests include instrumentation for hyperspectral imaging, regression and classification models for spectral data, and imaging fluorescence spectroscopy.

Spectroscopy has been an integral part of research at Nofima for decades, and research on hyperspectral imaging started in 1997. Nofima has also participated in numerous research projects with partners within the dairy, vegetable, poultry, meat, and fish industries. This has resulted in several publications, patents, and commercial products. The lecturers will give an overview both of their own work and of other relevant work performed at Nofima.

Seminar 5: Snapshot hyperspectral imaging: technology, promise, pitfalls

Content:
The past 5 years, imec has demonstrated several CMOS based spectral filter on-chip technologies, enabling a multitude of snapshot hyperspectral imaging sensor, camera and system solutions. This lecture will review these state of the art snapshot hyperspectral imaging technologies and their key applications. It will also discuss the possible trade-off’s and pitfalls to have in mind when integrating the sensors or cameras into systems.

Dr. Andy Lambrechts

Program Manager at IMEC

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Trainer profile:

Andy Lambrechts received a MS and Ph.D. degree in electrical engineering from Leuven University. He is Program Manager of the integrated imaging team and is working on hyperspectral imaging, lens-free microscopy and other activities that combine imec's process technology with systems and software knowledge to enable new applications in the vision domain.